Abstract
MicroRNAs (miRNAs) are one of the most important regulators of gene expression. Their biogenesis starts with the transcription of primary miRNA transcripts (pri-miRNAs). In plants, the RNase III enzyme DICER-LIKER 1 (DCL1), together with its partner, the double-stranded RNA-binding protein HYPONASTIC LEAVES 1 (HYL1), and the zinc-finger protein SERRATE (SE), process the imperfect miRNA-residing stem loop of pri-miRNAs to release the miRNA/miRNA∗ duplexes in the nucleus, which are then methylated by HUA1 ENHANCER 1 (Rogers and Chen, 2013; Song et al., 2019).
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