Forensic Features and Population Genetic Structure of Dong, Yi, Han, and Chuanqing Human Populations in Southwest China Inferred From Insertion/Deletion Markers.

Yubo Liu,Jinxing Ba,Qiyan Wang,Jiang Huang,Han Zhang,Jianxin Guo,Chuan-Chao Wang,Rong Hu,Lan-Hai Wei,Meiqing Yang,Jingyan Ji,Hongling Zhang,Jin Sun,Guanglin He,Xiaomin Yang,Dan Peng,Zheng Ren

doi:10.3389/fgene.2020.00360

Abstract

Guizhou province in southwest China has abundant genetic and cultural diversities, but the forensic features and genetic structure of Guizhou populations remain poorly understood due to the sparse sampling of present-day populations. Here, we present 30 insertion/deletion polymorphisms (InDels) data of 591 human individuals collected from four populations, Dong, Yi, Han, and Chuanqing residing in Guizhou. We calculated the forensic parameters of 30 InDel loci and found that this panel meets the efficiency of forensic personal identification based on the high combined power of discrimination, but it could only be used as a complementary tool in the parentage testing because of the lower combined probability of exclusion values. The studied populations are genetically closer related to geographically adjacent or linguistically related populations in southern China, such as the Tai-Kadai and Hmong-Mien speaking groups. The unrecognized ethnic Chuanqing people show an additional genetic affinity with Han Chinese, highlighting the role of possible military immigrations in their origin.

Highlights

Insertion/deletion polymorphisms (InDels) are biallelic length polymorphisms based on insertion or deletion of one or more nucleotides in the genome
We found that this panel meets the efficiency of forensic personal identification based on the high combined power of discrimination (>0.9999), but it could only be used as a complementary tool in the parentage testing because of the lower combined probability of exclusion values
We first investigated the forensic efficiency of the 30-InDel panel in Dong, Yi, Han, and Chuanqing residing in Guizhou, southeast China

Summary

Introduction

Insertion/deletion polymorphisms (InDels) are biallelic length polymorphisms based on insertion or deletion of one or more nucleotides in the genome. InDels are widely distributed across the human genome, with the total number estimated at approximately 2 million (Mills et al, 2011). InDels are considered to be ideal forensic markers with reduced amplicon size and low mutation rates (Sheng et al, 2018). InDels can be analyzed through simple PCR amplification and high-throughput electrophoresis, which are commonly used in forensic short tandem repeat (STR) analysis. InDels have been increasingly explored and used in forensic genetics (Arenas et al, 2017; Chen et al, 2019; Pan et al, 2019).

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