FORENSIC DNA PHENOTYPING FOR CRIMINAL IDENTIFICATION

Abstract

The purpose of the work – to carry out an analysis of modern achievements andpossibilities of forensic DNA phenotyping to determine the external characteristics ofa person, his biogeographical origin and age in order to facilitate criminal investigations.Conclusions. Forensic DNA Phenotyping (FDP) involves the prediction of a person’sexternal characteristics regarding appearance, biogeographic origin, and age based onDNA samples from a crime scene to support investigations and help to find unknowncriminals who cannot be identified through forensic STR profiling.DNA-based appearance prediction has moved beyond eye, hair and skin color andincluded new features such as eyebrow color, freckles, hair structure, male pattern hairloss and height. DNA-based biogeographic inference of ancestry has progressed fromcontinental ancestry to detecting subcontinental ancestry and resolving models of commonancestry in genetically mixed individuals. DNA-based age estimation is now possible notonly through blood testing, but also through somatic tissues such as saliva and bone, aswell as new markers and tools for analyzing semen. Technological advances have madeit possible to use forensic DNA technology with greatly increased multiplexing capabilityto simultaneously analyze hundreds of DNA predictors using targeted massively parallelsequencing (MPS). Forensically validated MPS-based FDP tools for DNA-based crimescene prediction are used to identify: several features of appearance; multiregional origin;several appearance features together with multiregional origin; age by different tissue types. Despite recent advances that are likely to increase the impact of FDP in criminal casesin the near future, bringing reliable prediction of appearance, ancestry, and age fromcrime scene DNA to the level of detail and accuracy, that forensic scientists may desire,requires further intensive scientific research, along with technical developments andforensic validation.