Forensic characteristics and population construction of two major minorities from southwest China revealed by a novel 37 Y-STR loci system.

Li Luo,Hao Zhang,Pengyu Chen,Min Li,Siyu Chai,Jian Yu,Chengtao Li,Xijie Hu,Yingnan Bian,Lilan Yao

doi:10.1098/rsos.210447

Abstract

Y-chromosome short tandem repeats (Y-STRs) have become important supplementary evidence in forensic science. Nowadays, the Y-chromosome STR haplotype reference database (YHRD) contains abundant Y-STR haplotype data from all over the world, while haplotype data of Guizhou Miao and Tujia are scarce. Hence, genetic polymorphisms of 37 Y-STRs were investigated in 446 unrelated males (206 Miao males and 246 Tujia males) residing in Guizhou Province. A total of 206 and 242 unique haplotypes with the highest diversity value of 0.9665 and 0.9470 were obtained. The heatmap, multidimensional scaling (MDS), the unweighted pair-group method with arithmetic means (UPGMA) tree and principal component analysis (PCA) based on the genetic distance (Rst) value within our studied populations and other 26 populations indicated that population structures follow the boundary of the continent. Guizhou Miao and Guizhou Tujia populations have intimate relationships with East Asian populations, especially the geographically close, similar history and the same language family populations.

Highlights

Y-chromosome short tandem repeats (Y-STRs), with the characteristics of male-specific, patroclinous and haplotype genetic, have been widely used in human evolution [1], genealogical research [2], population structure [3], forensic personal identification and paternity testing [4]
Haplotypes containing 37 Y-STRs from 206 Miao male individuals and 246 Tujia male individuals residing in Guizhou Province were obtained
A novel 37 Y-STR loci system was tested in the Miao and Tujia populations residing in Guizhou Province

Summary

Introduction

Y-chromosome short tandem repeats (Y-STRs), with the characteristics of male-specific, patroclinous and haplotype genetic, have been widely used in human evolution [1], genealogical research [2], population structure [3], forensic personal identification and paternity testing [4]. The largest of freely accessible online database named the Y-chromosome STR haplotype reference database (YHRD, https://yhrd.org, release 62) have been established. The YHRD website contains abundant Y-STR haplotype data from diverse populations and ethnic groups all over the world. There are fewer population data reports of Y-STRs in Guizhou Miao and Guizhou Tujia populations. The AGCU Y37 PCR amplification kit contains six-colour fluorescence, which can greatly improve the polymorphic information content and individual discrimination The AGCU Y37 PCR amplification kit contains six-colour fluorescence, which can greatly improve the polymorphic information content and individual discrimination (http://www. agcu.cn/page8?article_id=25&brd=1)

Methods

Results

Discussion

Conclusion