Force-velocity relationships in actin-myosin interactions causing cytoplasmic streaming in algal cells.

Abstract

Cytoplasmic streaming in giant internodal cells of green algae is caused by ATP-dependent sliding between actin cables fixed on chloroplast rows and cytoplasmic myosin molecules attached to cytoplasmic organelles. Its velocity (>/=50 micro m s(-1)) is many times larger than the maximum velocity of actin-myosin sliding in muscle. We studied kinetic properties of actin-myosin sliding causing cytoplasmic streaming in internodal cell preparations of Chara corallina, into which polystyrene beads, coated with cytoplasmic myosin molecules, were introduced. Constant centrifugal forces directed opposite to the bead movement were applied as external loads. The steady-state force-velocity (P-V) curves obtained were nearly straight, irrespective of the maximum isometric force generated by cytoplasmic myosin molecules, indicating a large duty ratio of cytoplasmic myosin head. The large velocity of cytoplasmic streaming can be accounted for, at least qualitatively, by assuming a mechanically coupled interaction between cytoplasmic myosin heads as well as a large distance of unitary actin-myosin sliding.