Abstract

We introduce a new device for measuring optically induced forces on microparticles and cells. It uses high-frequency electric field cages (octopoles); dielectrophoretic force calculations allow calibration of optical tweezers. A microsystem in the form of a microscope slide was fabricated by photolithography and electrically connected to a 4-phase high-frequency generator. The possibilities and advantages of a combined electro-optical trap are exemplified by the manipulation of latex beads and human erythrocytes. The system clearly detects misalignments of optical tweezers. In spite of instabilities it is possible to obtain a force-versus-power calibration. Further possibilities of the technique are discussed.

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