Forbs, graminoids, and lepidopterans: breadth and seasonal variation in the diet of the New Mexico jumping mouse (Zapus luteus)

Abstract

Abstract The New Mexico Jumping Mouse (Zapus luteus) is a federally endangered granivore active for only 3 to 5 months annually. Knowledge of diet can help guide habitat recovery and yet despite calls for restoration of its riparian habitat, the diet of Z. luteus remains largely unknown. To date, only 8 plant species have been described in the diet—whereas insectivory, mycophagy, and dietary shifts have only been hypothesized. In the late summer, Z. luteus rapidly accumulates fat for a 9-month hibernation and restoration may fail if seasonal variation in the diet is overlooked. We used DNA metabarcoding on fecal DNA (n = 165) to resolve dietary taxa within multiple trophic levels of the diet and investigate monthly patterns of consumption. Seldom studied in metabarcoding frameworks, we also investigated exogenous contamination in Sherman live-capture traps. Potential contamination among feces, pelage, and trap surfaces was detectable but low (median = 0% to 2%), with fecal communities distinct from exogenous sources. The diet of Z. luteus was varied and most frequently (frequency of occurrence) of graminoids, forbs, lepidopterans, dipterids, and false-truffles. For plant foods, dietary diversity increased throughout their season of activity, shifting from sedges and woody vegetation in the early season (June to July) to forbs and grasses in the late season (August to September). Insect consumption was consistently detected throughout the season, whereas mycophagy was more frequently detected in August, when hypogeous fungi are typically more abundant. The breadth of dietary taxa suggests plasticity in resource use, potentially accommodating diverse patterns of seed availability throughout their active period. Shifts in plant consumption may reflect a dietary adaptation to the phenological patterns of their plant communities, a synchrony that might benefit both pre- and post-hibernation survival. Our work substantially improves our understanding of the diet of Z. luteus (241 dietary taxa) and will be useful for guiding habitat recovery. It also offers scalable methods to further investigate the diet under disturbance contexts (e.g., grazing, wildfire, drought).