Forage crops alter soil bacterial and fungal communities in an apple orchard

Abstract

ABSTRACTIn this study, we investigated the effects of planting three types of forage crops in an apple orchard on the soil microbial community structure. The apple orchard was intercropped with native grasses (NG), red clover (RC; Trifolium pratense L.), ryegrass (RE; Trifolium repens L.), and no vegetation (CT control; clean tillage). We obtained soil samples from depths of 0–20, 20–40, and 40–60 cm from the different treatment plots in the orchard and analysed them using a high-throughput DNA sequencing technique. The three forage crops had affected the soil bacterial and fungal community structures. Compared with CT control, intercropping with NG increased the proportion of Acidobacteria and reduced those of Nitrospirae and Verrucomicrobia, whereas intercropping with RC increased the proportions of Nitrospirae and Verrucomicrobia and reduced that of Planctomycetes. Intercropping with RE increased the proportions of Nitrospirae and Chloroflexi, whereas reduced that of Acidobacteria. Furthermore, unlike in the other treatments, intercropping with NG increased the proportion of Zygomycota in the 0–20-cm soil layer. Intercropping with RC increased the proportion of Chytridiomycota in all the three soil layers, whereas intercropping with RE increased the proportion of Basidiomycota in the 20–40-cm soil layer. Collectively, these findings suggest that intercropping with forage crops, especially RC, in an apple orchard, could alter soil microbial community structure. In our previous study, we showed that microbial sole-carbon-source utilisation is changed by intercropping with forage crops; thus, it can be considered as an effective approach to improve the efficiency of soil C cycling in the apple orchard by altering the microbial community structure.