Foot-and-mouth disease virus-induced alterations of baby hamster kidney cell macromolecular biosynthesis: inhibition of ribonucleic acid methylation and stimulation of ribonucleic acid synthesis.

Abstract

The kinetics of ribonucleic acid (RNA) and protein synthesis and RNA methylation were examined after foot-and-mouth disease virus (FMDV) infection of baby hamster kidney cells. The synthesis of RNA extracted from the whole cells was stimulated two- to threefold above the control level of synthesis. This increased rate was attributed to viral RNA synthesis. The inhibition of host RNA methylation was concomitant with but more pronounced than protein synthesis inhibition. The methylation of transfer RNA was initially inhibited by virus infection, but rose to within 70 to 80% of the control level just prior to the production of maximal amounts of virus-specific RNA polymerase. Cycloheximide studies showed that rapid cessation of protein synthesis did not result in the immediate cessation of RNA methylation. A comparison between the kinetics of inhibition of these processes by cycloheximide and FMDV infection suggests that FMDV selectively inhibits RNA methylation.