Abstract
Using a nutrient enrichment experiment in an Andean mountain stream , we used stable isotope ratios ( 15 N and 13 C) to analyze different trophic compartments: 1) basal level: CPOM and biofilm; 2) primary consumers – macroinvertebrates: collector-gatherers( Heterelmis sp, Thraulodes sp and Trichorythodes sp), and collector-filterers ( Simulium sp); 3) predators – fish ( Oncorhynchusmykiss and Trichomycterusbogotensis). The average fractionation of nitrogen among the primary consumers with respect to CPOM was 4.7‰ and 1.7‰ with respect to biofilm. Predators incrementedtheir 15 N signalby 5.9% with respect to primary consumers . A depletion of 15 N was observed in Impact with respect tocontrol reach after fertilization in different compartments (biofilm, Heterelmis sp . , Simulium sp. and Tricorythodes sp .) , while depletion was not significant for top predators. In most cases, the 13 C signal of biofilm overlapped with that of primary consumers, but a clear enrichment was observed with respect to CPOM. The macroinvertebrates referred to were selected to analyze their gut content and the results showed us that fine detritus is the most abundant food in invertebrates, and only Heterelmis sp. showed significant differences in fine detritus and vegetal matter between control and impact reaches after the nutrient addition.
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