Food web biomagnification of the neurotoxin β-N-methylamino-L-alanine in a diatom-dominated marine ecosystem in China

Abstract

The neurotoxin β-N-methylamino-L-alanine (BMAA) reported in some cyanobacteria and eukaryote microalgae is a cause of concern due to its potential risk of human neurodegenerative diseases. Here, BMAA distribution in phytoplankton, zooplankton, and other marine organisms was investigated in Jiaozhou Bay, China, a diatom-dominated marine ecosystem, during four seasons in 2019. Results showed that BMAA was biomagnified in the food web from phytoplankton to higher trophic levels. Trophic magnification factors (TMFs) for zooplankton, bivalve mollusks, carnivorous crustaceans and carnivorous gastropod mollusks were ca. 4.58, 30.1, 42.5, and 74.4, respectively. Putative identification of β-amino-N-methylalanine (BAMA), an isomer of BMAA, was frequently detected in phytoplankton samples. A total of 56 diatom strains of the genera Pseudo-nitzschia, Thalassiosira, Chaetoceros, Planktoniella, and Minidiscus isolated from the Chinese coast were cultured in the laboratory, among which 21 strains contained BMAA mainly in precipitated bound form at toxin concentrations ranging from 0.11 to 3.95 µg/g dry weight. Only 2,4-diaminobutyric acid (DAB) but not BMAA or BAMA was detected in seven species of bacteria isolated from the gut of gastropod Neverita didyma, suggesting that this benthic vector of BMAA may have accumulated this compound via trophic transfer.