Abstract
An inexpensive chromogenic enzymatic detection system was developed for the assay of lactose in foods using a microassay format. This assay system is based on the actions of two substrate-specific enzymes, lactase and glucose oxidase; which, when combined in a buffered solution of iodide, molybdate and polyvinyl alcohol, produce a coloured iodine-polyvinyl alcohol complex in the presence of lactose. The enzymatic reactions were performed in the wells of a microtitre plate and measured using a microtitre plate reader. The enzymatic detection system exhibited a linear dose-response relationship with respect to lactose concentration. The application of this detection system as a screening method for lactose in grated parmesan cheese samples is demonstrated.
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