Food reward induction of rhythmic clock gene expression in the prefrontal cortex of rats is accompanied by changes in miR‐34a‐5p expression

Abstract

The current study is focused on mechanisms by which the peripheral circadian oscillator in the prefrontal cortex (PFC) participates in food reward-induced activity. The experimental group of male Wistar rats was trained to receive a food reward with a low hedonic and caloric value. Afterwards, animals were exposed to a 5h phase advance. Experimental animals could access a small food reward as they had been accustomed to, while control rats were exposed to the same phase shift without access to a food reward. When synchronisation to a new light:dark cycle was accompanied by intake of food reward, animals exerted more exact phase shift compared to the controls. In rats with access to a food reward, a rhythm in dopamine receptors types 1 and 2 in the PFC was detected. Rhythmic clock gene expression was induced in the PFC of rats when a food reward was provided together with a phase shift. The per2 and clock genes are predicted targets of miR-34a-5p. The precursor form of miR-34a-5p (pre-miR-34a-5p) showed a daily rhythm in expression in the PFC of the control and experimental groups. On the other hand, the mature form of miR-34a-5p exerted an inverted rhythm compared to pre-miR-34a-5p and negative correlation with per and clock genes expression only in the PFC of rewarded rats. A difference in the pattern of mature and pre-miR-34a-5p values was not related to expression of enzymes drosha, dicer and dgcr8. A role of the clock genes and miR-34a-5p in reward-facilitated synchronisation has been hypothesised.