Food availability outweighs ocean acidification effects in juvenile Mytilus edulis: laboratory and field experiments

Abstract

Ocean acidification is expected to decrease calcification rates of bivalves. Nevertheless, in many coastal areas high pCO2 variability is encountered already today. Kiel Fjord (Western Baltic Sea) is a brackish (12-20gkg(-1) ) and CO2 enriched habitat, but the blue mussel Mytilus edulis dominates the benthic community. In a coupled field and laboratory study we examined the annual pCO2 variability in this habitat and the combined effects of elevated pCO2 and food availability on juvenile M.edulis growth and calcification. In the laboratory experiment, mussel growth and calcification were found to chiefly depend on food supply, with only minor impacts of pCO2 up to 3350μatm. Kiel Fjord was characterized by strong seasonal pCO2 variability. During summer, maximal pCO2 values of 2500μatm were observed at the surface and >3000μatm at the bottom. However, the field growth experiment revealed seven times higher growth and calcification rates of M.edulis at a high pCO2 inner fjord field station (mean pCO2 ca. 1000μatm) in comparison to a low pCO2 outer fjord station (ca. 600μatm). In addition, mussels were able to out-compete the barnacle Amphibalanus improvisus at the high pCO2 site. High mussel productivity at the inner fjord site was enabled by higher particulate organic carbon concentrations. Kiel Fjord is highly impacted by eutrophication, which causes bottom water hypoxia and consequently high seawater pCO2 . At the same time, elevated nutrient concentrations increase the energy availability for filter feeding organisms such as mussels. Thus, M.edulis can dominate over a seemingly more acidification resistant species such as A.improvisus. We conclude that benthic stages of M.edulis tolerate high ambient pCO2 when food supply is abundant and that important habitat characteristics such as species interactions and energy availability need to be considered to predict species vulnerability to ocean acidification.