Food Allergen Detection Methods: A Coordinated Approach
The levels (1-2%) and increasing severity of allergic responses to food in the adult population are well documented, as is the phenomenon of even higher (3-8%) and apparently increasing incidence in children, albeit that susceptibility decreases with age. Problematic foods include peanut, milk, eggs, tree nuts, and sesame, but the list is growing as awareness continues to rise. The amounts of such foods that can cause allergic reactions is difficult to gauge; however, the general consensus is that ingestion of low parts per million is sufficient to cause severe reactions in badly affected individuals. Symptoms can rapidly-within minutes-progress from minor discomfort to severe, even life-threatening anaphylactic shock in those worst affected. Given the combination of high incidence of atopy, potential severity of response, and apparently widespread instances of "hidden" allergens in the food supply, it is not surprising that this issue is increasingly subject to legislative and regulatory scrutiny. In order to assist in the control of allergen levels in foods to acceptable levels, analysts require a combination of test methods, each designed to produce accurate, timely, and cost-effective analytical information. Such information contributes significantly to Hazard Analysis Critical Control Point programs to determine food manufacturers' risk and improves the accuracy of monitoring and surveillance by food industry, commercial, and enforcement laboratories. Analysis thereby facilitates improvements in compliance with labeling laws with concomitant reductions in risks to atopic consumers. This article describes a combination of analytical approaches to fulfill the various needs of these 3 analytical communities.
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- Allergy
- Research Article
120
- 10.1021/jf902694j
- Jan 27, 2010
- Journal of Agricultural and Food Chemistry
As peanut allergy is an increasing public health risk, affecting over 1% of the United States and United Kingdom school children, it is important that methods and reagents for accurate diagnosis of food allergy and detection of allergenic foods are reliable and consistent. Given that most current experimental, diagnostic, and detection tests rely on the presence of soluble allergens in food extracts, we investigated the effects of thermal processing on the solubility and IgE binding of the major peanut allergens, Ara h 1 and Ara h 2. The soluble and insoluble fractions of peanuts that were boiled, fried, and roasted were subjected to electrophoresis and Western blot analysis using anti-Ara h 1 and anti-Ara h 2 antibodies and serum IgE from peanut allergic individuals. Overall protein solubility is reduced with processing and IgE binding increases in the insoluble fractions, due mostly to the increase in the amount of insoluble proteins, with increased time of heating in all processes tested. Therefore, it can be concluded that thermal processing of peanuts alters solubility, and the differences in protein solubility within various extract preparations may contribute to inconsistent skin prick test and immunoassay results, particularly when nonstandardized reagents are used.
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13
- 10.1016/j.lwt.2015.01.023
- Jan 23, 2015
- LWT - Food Science and Technology
Development of real-time PCR assays to detect cashew (Anacardium occidentale) and macadamia (Macadamia intergrifolia) residues in market analysis of processed food products
- Book Chapter
5
- 10.5772/17858
- Sep 12, 2011
As a major food ingredient, soybean provides numerous health benefits, for example, individuals who consume soybean-rich diets exhibit lower incidence of high plasma cholesterol, cancer (including bowel and kidney), diabetes mellitus, and obesity (Carroll and Kurowska, 1995; Friedman and Brandon, 2001; Duranti et al., 2004; Ali et al., 2004; Omini and Aluko, 2005; Kim et al., 2006). However, soybean is also among the “big 8” most allergenic foods, and the only possible solution currently to prevent allergenic reactions is total avoidance of the allergen-containing foods. Because soybean is ubiquitous in vegetarian and many meat based food products, avoidance has become increasingly difficult, and its prevalence will inevitably continue to rise. Due to the innumerable health benefits and economic importance of soy commercial products, there is a mounting need to remove the allergenic components contained in soybean proteins to a threshold deemed to be safe. The estimated threshold level for common food allergy is usually low and a small amount of the allergen may be enough to trigger an allergenic reaction (Poms et al., 2004). The threshold for soybean allergen is estimated to range from 88 mg to several grams of soy protein (Bindslev-Jensen et al., 2002; Fiocchi et al., 2003). Soybean allergy is of particular importance because soybean is widely used in processed foods and represents a particularly insidious source of hidden allergens. Since finding foods that do not contain soy is difficult and total elimination of food allergens is practically impossible to attain, investigations on the hypoallergenization of soy ingredients and products are imperative. Current requirements by the labeling regulations also make it imperative to identify a processing technology that is capable of reducing or eliminating the allergens from soy containing products. Elimination or reduction of allergens in allergenic foods has been attempted for years by various investigators, which has included, among various strategies, the use of genetic engineering, thermal processing, enzyme treatment, ultrafiltration, chemical agents, microwave, irradiation, high pressure processing, pulsed ultraviolet light, power ultrasound and pulsed electric field. The reduction or elimination of allergens from soybean proteins by different processing technologies offers unique insight to the structure and biological interaction of the antigenic proteins. With allergens reduced, the industry can further profit from the economical attributes of soybean and promote the
- Research Article
43
- 10.1111/j.1365-2621.2005.tb11454.x
- Aug 1, 2005
- Journal of Food Science
ABSTRACTFor various reasons, a considerable majority of the global population must rely on plant proteins obtained from cereals, legumes (including oilseeds), fruits, vegetables, and nuts to satisfy dietary protein needs and requirements. Edible seeds are a significant source of proteins in livestock production and in the manufacture of pet foods. In addition, edible seeds are important sources of carbohydrates (including dietary fiber), minerals, and certain vitamins in human and animal food supply. For various reasons, edible seeds are underutilized as human food. To fully exploit this renewable natural resource to its full potential, focused research efforts are warranted. With increased number of seed proteins being identified as food allergens, renewed interest in seed proteins is evident. In this article, a brief overview of seed proteins with special reference to their allergenicity is provided. An attempt is made to identify areas needing further research.
- Book Chapter
2
- 10.1002/9781118519219.ch06
- Dec 27, 2013
Detection of plant allergens in foods
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184
- 10.1016/j.foodchem.2007.11.013
- Nov 17, 2007
- Food Chemistry
Immunoreactivity reduction of soybean meal by fermentation, effect on amino acid composition and antigenicity of commercial soy products
- Research Article
116
- 10.1016/j.tifs.2010.02.003
- Mar 6, 2010
- Trends in Food Science & Technology
Immunochemical and DNA-based methods in food allergen analysis and quality assurance perspectives
- Research Article
222
- 10.1007/s00217-005-0178-8
- Feb 15, 2006
- European Food Research and Technology
Cow's milk allergy (CMA) is one of the most common food allergies in childhood. This allergy is normally outgrown in the first year of life, however 15% of allergic children remain allergic. Many studies have been carried out to define and characterise the allergens involved in CMA and described two major allergens: casein (αs1-CN) and β-lactoglobulin. In addition to this, many other milk proteins are antigenic and capable of inducing immune responses. Milk from sheep or goats differs from cow's milk (CM) in terms of composition and allergenic properties. Food processing such as heating affects the stability, structure and intermolecular interactions of CM proteins, thereby changing the allergenic capacity. Chemical and proteolytic treatments of milk to obtain milk hydrolysates have been developed to reduce allergic reactions. Prevention of CMA largely relies on avoidance of all food products containing cow's milk. To achieve this, interest has focused on the development of various technologies for detecting and measuring the presence of milk allergens in food products by immunoassays or proteomic approaches. This review describes the technologies implemented for the analysis of milk allergens (allergenicity, biochemistry) as well as their potential detection in food matrices.
- Research Article
37
- 10.1007/s12161-008-9023-6
- Mar 19, 2008
- Food Analytical Methods
Appropriate detection methods have to be provided to assure the compliance with the recently established regulatory provisions concerning the labeling of allergens in food. Therefore, a novel real-time polymerase chain reaction (PCR) system for the specific and sensitive detection of cashew nut (Anacardium occidentale) was developed. Specificity was checked against DNA from 56 plant and animal species to avoid cross-reactivity to phylogenetically related and other food-relevant organisms. The absolute limit of detection (LOD) was determined to be 0.5 pg genomic cashew DNA and 10 copies, respectively, and the practical LOD examined exemplarily for pesto Genovese was 2 mg/kg. In addition, analysis of different retail samples was performed to demonstrate the suitability of the new assay for manifold applications.
- Research Article
4
- 10.5539/jfr.v8n3p71
- Apr 16, 2019
- Journal of Food Research
Food allergens are a well acknowledged issue in food industry and are regulated by legislation. The presence of allergens can either origin from the raw material or due to contamination during production. Allergen information on packaging is mandatory although it cannot be accurate in the case of contamination therefore warnings are used. The purpose of the study is the development and validation of a SYBR Green Real Time Polymerase Chain Reaction method using specific primer pairs based on Jug r 1, Jug r 3, and Jug r 4 allergen-coding sequences to improve the sensitivity of Real Time Polymerase Chain Reaction techniques for detection of walnut and almond traces in commercial food products and its comparison with ELISA methodology in terms of detection ability. A total of 100 samples were collected from local markets and were analyzed by Real Time Polymerase Chain Reaction (RT-PCR) and ELISA methods. The results indicated that 16 samples (16%) were found positive in walnut traces and 18 samples (18%) were found positive in almond traces by Real Time Polymerase Chain Reaction of which Elisa identified 14 samples (14%) positive in walnut traces and 15 samples (15%) positive in almond traces. Among them, 4 samples (25%) that contained walnut traces and 6 samples (33.3%) that contained almond traces had no allergen declaration on their label. The improved accuracy of Real Time Polymerase Chain Reaction underlines the importance of this method for allergen detection and quantification in the food industry
- Research Article
40
- 10.1089/fpd.2010.0809
- May 13, 2011
- Foodborne pathogens and disease
Hazard analysis critical control point (HACCP) programs have been endorsed and implemented globally to enhance food safety. Our objective was to identify, assess, and summarize or synthesize the published research investigating the effect of HACCP programs on microbial prevalence and concentration on food animal carcasses in abattoirs through primary processing. The results of microbial testing pre- and post-HACCP implementation were reported in only 19 studies, mostly investigating beef (n=13 studies) and pork (n=8 studies) carcasses. In 12 of 13 studies measuring aerobic bacterial counts, reductions were reported on beef (7/8 studies), pork (3/3), poultry (1/1), and sheep (1/1). Significant (p<0.05) reductions in prevalence of Salmonella spp. were reported in studies on pork (2/3 studies) and poultry carcasses (3/3); no significant reductions were reported on beef carcasses (0/8 studies). These trends were confirmed through meta-analysis of these data; however, powerful meta-analysis was precluded because of an overall scarcity of individual studies and significant heterogeneity across studies. Australia reported extensive national data spanning the period from 4 years prior to HACCP implementation to 4 years post-HACCP, indicating reduction in microbial prevalence and concentration on beef carcasses in abattoirs slaughtering beef for export; however, the effect of abattoir changes initiated independent of HACCP could not be excluded. More primary research and access to relevant proprietary data are needed to properly evaluate HACCP program effectiveness using modeling techniques capable of differentiating the effects of HACCP from other concurrent factors.
- Research Article
20
- 10.4315/0362-028x-62.8.887
- Aug 1, 1999
- Journal of Food Protection
Verifying Apple Cider Plant Sanitation and Hazard Analysis Critical Control Point Programs: Choice of Indicator Bacteria and Testing Methods
- Research Article
9
- 10.1081/fri-120014687
- Jan 10, 2002
- Food Reviews International
A hazard analysis critical control point (HACCP) program itself is a straightforward and logical system of enhancing food safety through the prevention of problems. This paper focuses on the role of each step in HACCP programs. Condensed milk products, which have wide application in many sectors of the food industry, are relatively short shelf-life products. Therefore, HACCP analysis of this product is an important issue for public health. Implementation of the HACCP system to production of bulk-condensed milk proved to be a valuable tool for improving the safety and quality characteristics of that product. Chemical and microbiological test results, in addition to the other programs, play a lead role in developing monitoring strategies and generating validation data to help the HACCP team assess the effectiveness of the processing controls being used.
- Research Article
58
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- Jan 1, 2003
- Journal of the American Dietetic Association
Implementation of HACCP and prerequisite programs in school foodservice
- Research Article
27
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Predictive Microbiology and HACCP
- Research Article
- 10.1300/j030v07n03_02
- Jun 3, 1998
- Journal of Aquatic Food Product Technology
The Hazard Analysis Critical Control Point (HACCP) concept can best be described as a preventive system of control based on a series of steps taken to ensure the safety of new and processed seafood. It is a systematic approach to minimize or prevent consumer hazards which can include, according to which regulatory scheme you ascribe to, food safety, plant and food hygiene and product integrity. The HACCP approach is a seven-step system designed to assess hazards associated with potentially hazardous foods from point of receipt to shipment of finished product. The HACCP concept for inspecting seafood was recommended by the U.S. National Academy of Sciences as the desired program to redefine consumer protection. HACCP provides both the operator and inspector a total view of the process and identifies steps “critical to safety.” To review and set the stage for later discussions on the future implications and regulatory oversight of this mandatory system, the seven steps of HACCP must be carefully considered. While HACCP systems must be designed by individual manufacturers and tailored to their individual processing and distribution requirements. HACCP systems are designed and function in a manner consistent with the stated goal of preventing potentially hazardous products from reaching consumers. Having established the basis of HACCP, this paper will discuss concerns over inspection training, export certification, future research needs, the hazards and control guide, equivalency, memorandums of understanding, relationship with the National Marine Fisheries Service, appeals, non-regulatory review and federal measurement of the results of HACCP implementation. Will HACCP experiences with seafood lead to similar programs for other components of the U.S. food supply?
- Research Article
25
- 10.1080/87559129509541062
- Nov 1, 1995
- Food Reviews International
Hazard analysis critical control point (HACCP) is a food process control system developed in the early 1970s to ensure the safety of foods for the United States space program. Since the 1970s HACCP has evolved into a recognized means to assure the safety of foods throughout the food industry both within the United States and elsewhere. Based on the principle of prevention rather than detection, HACCP has been extensively and successfully used in the low‐acid canned food industry since the early 1970s. Since that time, HACCP has achieved greater prominence with a refinement of the HACCP principles and the application of HACCP to other processes and products. The purpose of this review is to trace the evolution of HACCP to its present‐day applications in the food industry and discuss its importance for the production of a safer food supply.
- Research Article
57
- 10.1016/j.foodcont.2004.09.010
- Nov 20, 2004
- Food Control
Implementation of HACCP and prerequisite programs in food businesses in Turkey
- Conference Article
6
- 10.1109/rams.1994.291077
- Jan 24, 1994
Food processors are being challenged to increase product safety while improving quality and productivity. These objectives can be achieved using total quality management (TQM) and hazard analysis critical control point (HACCP) programs. HACCP embodies many of the criteria of TQM. These include: customer satisfaction; interaction between organizational components; information feedback; documentation; auditing; management commitment and leadership; teamwork and trust between management and employees; and empowerment. HACCP is preventive and not after-the-fact testing dependent. HACCP programs have been implemented with varying degrees of success. Failure usually has been caused by not differentiating between critical control points (CCP's) and control points (CP's). CP's must be controlled because of the need to anticipate and prevent potential regulatory and consumer-quality problems. The development of separate programs for these elements permits effective control without impacting on productivity.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
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15
- 10.1111/j.1365-2621.2007.01619.x
- Jul 14, 2008
- International Journal of Food Science & Technology
SummaryThis paper examines the factors, which influence the safety characteristics of Tarhana (Tarhana is a fermented, dried dough product commonly reconstituted and served as a hot soup). Since Tarhana is becoming an important part of the modern diet in many communities. The objectives of this study were to determine food safety practices and procedures related to the hazard analysis critical control point programme. The significant hazards found in hazard analysis were used for determination of risk assessment by five‐class hazard score matrix. The study revealed that the Critical Control Points (CCPs) for Tarhana production were cooking, fermentation, drying and sieving. At the same time, critical factors and limits for CCPs were determined. Preventative and corrective actions were proposed.
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18
- 10.3168/jds.s0022-0302(00)75199-x
- Dec 1, 2000
- Journal of Dairy Science
Controlling On-Farm Inventories of Bulk-Tank Raw Milk— An Opportunity to Protect Public Health
- Front Matter
3
- 10.1016/j.jaip.2018.11.035
- Feb 1, 2019
- The Journal of Allergy and Clinical Immunology: In Practice
The Evolving Science of Peanut Allergy
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- 10.4081/ijfs.2023.11109
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- Italian Journal of Food Safety
Dry aging is a process during which meat is stored within maturation chambers at low temperatures and low relative humidity, resulting in improved tenderness and flavor development. The cuts are exposed to the atmosphere by hanging them or setting them on racks in the maturation chamber without any protective packaging. Animals and humans are usually the major sources of bacterial food contamination in the meat industry, but other routes might be involved. Therefore, procedures to reduce or eliminate pathogens from surfaces are crucial for an effective hazard analysis critical control point program in the food industry and other environments. This study aimed to assess the survival of Listeria monocytogenes, Escherichia coli, Salmonella spp., and Staphylococcus aureus on the inner surface of dry aging chambers. Moreover, we tested the efficacy of alkaline electrolyzed water (REW) for its application within a procedure aimed at reducing foodborne pathogens during meat storage. Environmental conditions inside the dry aging cabinet determine a reduction of circa 3 log CFU/cm2 of the considered microorganisms on the inner surface in 24 hours. Additionally, the nebulization of REW with the smoking system increased the count reduction in 24 hours due to environmental conditions for L. monocytogenes (~1 log CFU/cm2) and for S. aureus (~2 log CFU/cm2). In this context, the use of REW can be justified for routine cleaning procedures of the surfaces, with the added value of being safe to handle, not containing environmental pollutants, and making it unnecessary to rinse surfaces due to its instability.
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14
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Possession of epinephrine auto-injectors by Canadians with food allergies
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18
- 10.1108/09526860610671409
- Jun 1, 2006
- International Journal of Health Care Quality Assurance
Hospital cleaning and healthcare associated infections (HCAIs) continue to attract adverse media attention and consumer concern. Parallels exist with similar publicity relating to cleaning and food safety in the food industry almost 13 years earlier. This paper examines some of the management solutions developed in the food industry, and discusses their application to healthcare delivery. The food industry is managing food safety by adopting a dual approach based on pre-requisite programmes and Hazard Analysis Critical Control Points (HACCP). How these differ is described and how the approaches and terminology can be adapted for use in healthcare is discussed. The food industry is moving towards external certification of safety using national and international standards. The HACCP approach, a management tool and a central requirement of these standards, is evolving and there is interest worldwide from the healthcare community. Its application to the decontamination of endoscopes, using conventional HACCP, is presented, as well as suggestions for a simplified format for managing patient-related procedures. Taking this type of approach to the management of HCAIs could provide greater transparency, reduce infection rates and increase consumer confidence. Potential problems in adopting HACCP, including cost and human resource, are discussed. The HACCP method/approach has previously been mentioned in the medical literature but this paper is one of the few to examine, from basic principles, its infection control application within a broader approach to quality assurance.
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