Food Allergen Detection Methods: A Coordinated Approach

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The levels (1-2%) and increasing severity of allergic responses to food in the adult population are well documented, as is the phenomenon of even higher (3-8%) and apparently increasing incidence in children, albeit that susceptibility decreases with age. Problematic foods include peanut, milk, eggs, tree nuts, and sesame, but the list is growing as awareness continues to rise. The amounts of such foods that can cause allergic reactions is difficult to gauge; however, the general consensus is that ingestion of low parts per million is sufficient to cause severe reactions in badly affected individuals. Symptoms can rapidly-within minutes-progress from minor discomfort to severe, even life-threatening anaphylactic shock in those worst affected. Given the combination of high incidence of atopy, potential severity of response, and apparently widespread instances of "hidden" allergens in the food supply, it is not surprising that this issue is increasingly subject to legislative and regulatory scrutiny. In order to assist in the control of allergen levels in foods to acceptable levels, analysts require a combination of test methods, each designed to produce accurate, timely, and cost-effective analytical information. Such information contributes significantly to Hazard Analysis Critical Control Point programs to determine food manufacturers' risk and improves the accuracy of monitoring and surveillance by food industry, commercial, and enforcement laboratories. Analysis thereby facilitates improvements in compliance with labeling laws with concomitant reductions in risks to atopic consumers. This article describes a combination of analytical approaches to fulfill the various needs of these 3 analytical communities.

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CitationsShowing 10 of 37 papers
  • Research Article
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Processing Can Alter the Properties of Peanut Extract Preparations
  • Jan 27, 2010
  • Journal of Agricultural and Food Chemistry
  • David A Schmitt + 4 more

As peanut allergy is an increasing public health risk, affecting over 1% of the United States and United Kingdom school children, it is important that methods and reagents for accurate diagnosis of food allergy and detection of allergenic foods are reliable and consistent. Given that most current experimental, diagnostic, and detection tests rely on the presence of soluble allergens in food extracts, we investigated the effects of thermal processing on the solubility and IgE binding of the major peanut allergens, Ara h 1 and Ara h 2. The soluble and insoluble fractions of peanuts that were boiled, fried, and roasted were subjected to electrophoresis and Western blot analysis using anti-Ara h 1 and anti-Ara h 2 antibodies and serum IgE from peanut allergic individuals. Overall protein solubility is reduced with processing and IgE binding increases in the insoluble fractions, due mostly to the increase in the amount of insoluble proteins, with increased time of heating in all processes tested. Therefore, it can be concluded that thermal processing of peanuts alters solubility, and the differences in protein solubility within various extract preparations may contribute to inconsistent skin prick test and immunoassay results, particularly when nonstandardized reagents are used.

  • Research Article
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Development of real-time PCR assays to detect cashew (Anacardium occidentale) and macadamia (Macadamia intergrifolia) residues in market analysis of processed food products
  • Jan 23, 2015
  • LWT - Food Science and Technology
  • Inés María López-Calleja + 4 more

Development of real-time PCR assays to detect cashew (Anacardium occidentale) and macadamia (Macadamia intergrifolia) residues in market analysis of processed food products

  • Open Access Icon
  • Book Chapter
  • Cite Count Icon 5
  • 10.5772/17858
Soybean Allergens: Presence, Detection and Methods for Mitigation
  • Sep 12, 2011
  • Weihua Wade + 3 more

As a major food ingredient, soybean provides numerous health benefits, for example, individuals who consume soybean-rich diets exhibit lower incidence of high plasma cholesterol, cancer (including bowel and kidney), diabetes mellitus, and obesity (Carroll and Kurowska, 1995; Friedman and Brandon, 2001; Duranti et al., 2004; Ali et al., 2004; Omini and Aluko, 2005; Kim et al., 2006). However, soybean is also among the “big 8” most allergenic foods, and the only possible solution currently to prevent allergenic reactions is total avoidance of the allergen-containing foods. Because soybean is ubiquitous in vegetarian and many meat based food products, avoidance has become increasingly difficult, and its prevalence will inevitably continue to rise. Due to the innumerable health benefits and economic importance of soy commercial products, there is a mounting need to remove the allergenic components contained in soybean proteins to a threshold deemed to be safe. The estimated threshold level for common food allergy is usually low and a small amount of the allergen may be enough to trigger an allergenic reaction (Poms et al., 2004). The threshold for soybean allergen is estimated to range from 88 mg to several grams of soy protein (Bindslev-Jensen et al., 2002; Fiocchi et al., 2003). Soybean allergy is of particular importance because soybean is widely used in processed foods and represents a particularly insidious source of hidden allergens. Since finding foods that do not contain soy is difficult and total elimination of food allergens is practically impossible to attain, investigations on the hypoallergenization of soy ingredients and products are imperative. Current requirements by the labeling regulations also make it imperative to identify a processing technology that is capable of reducing or eliminating the allergens from soy containing products. Elimination or reduction of allergens in allergenic foods has been attempted for years by various investigators, which has included, among various strategies, the use of genetic engineering, thermal processing, enzyme treatment, ultrafiltration, chemical agents, microwave, irradiation, high pressure processing, pulsed ultraviolet light, power ultrasound and pulsed electric field. The reduction or elimination of allergens from soybean proteins by different processing technologies offers unique insight to the structure and biological interaction of the antigenic proteins. With allergens reduced, the industry can further profit from the economical attributes of soybean and promote the

  • Research Article
  • Cite Count Icon 43
  • 10.1111/j.1365-2621.2005.tb11454.x
Advances in Seed Protein Research: A Perspective on Seed Allergens
  • Aug 1, 2005
  • Journal of Food Science
  • Shridhar K Sathe + 2 more

ABSTRACTFor various reasons, a considerable majority of the global population must rely on plant proteins obtained from cereals, legumes (including oilseeds), fruits, vegetables, and nuts to satisfy dietary protein needs and requirements. Edible seeds are a significant source of proteins in livestock production and in the manufacture of pet foods. In addition, edible seeds are important sources of carbohydrates (including dietary fiber), minerals, and certain vitamins in human and animal food supply. For various reasons, edible seeds are underutilized as human food. To fully exploit this renewable natural resource to its full potential, focused research efforts are warranted. With increased number of seed proteins being identified as food allergens, renewed interest in seed proteins is evident. In this article, a brief overview of seed proteins with special reference to their allergenicity is provided. An attempt is made to identify areas needing further research.

  • Book Chapter
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  • 10.1002/9781118519219.ch06
Detection of plant allergens in foods
  • Dec 27, 2013
  • Dimosthenis Kizis

Detection of plant allergens in foods

  • Research Article
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  • 10.1016/j.foodchem.2007.11.013
Immunoreactivity reduction of soybean meal by fermentation, effect on amino acid composition and antigenicity of commercial soy products
  • Nov 17, 2007
  • Food Chemistry
  • Y.-S Song + 4 more

Immunoreactivity reduction of soybean meal by fermentation, effect on amino acid composition and antigenicity of commercial soy products

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  • 10.1016/j.tifs.2010.02.003
Immunochemical and DNA-based methods in food allergen analysis and quality assurance perspectives
  • Mar 6, 2010
  • Trends in Food Science & Technology
  • Linda Monaci + 1 more

Immunochemical and DNA-based methods in food allergen analysis and quality assurance perspectives

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Milk allergens, their characteristics and their detection in food: A review
  • Feb 15, 2006
  • European Food Research and Technology
  • Linda Monaci + 3 more

Cow's milk allergy (CMA) is one of the most common food allergies in childhood. This allergy is normally outgrown in the first year of life, however 15% of allergic children remain allergic. Many studies have been carried out to define and characterise the allergens involved in CMA and described two major allergens: casein (αs1-CN) and β-lactoglobulin. In addition to this, many other milk proteins are antigenic and capable of inducing immune responses. Milk from sheep or goats differs from cow's milk (CM) in terms of composition and allergenic properties. Food processing such as heating affects the stability, structure and intermolecular interactions of CM proteins, thereby changing the allergenic capacity. Chemical and proteolytic treatments of milk to obtain milk hydrolysates have been developed to reduce allergic reactions. Prevention of CMA largely relies on avoidance of all food products containing cow's milk. To achieve this, interest has focused on the development of various technologies for detecting and measuring the presence of milk allergens in food products by immunoassays or proteomic approaches. This review describes the technologies implemented for the analysis of milk allergens (allergenicity, biochemistry) as well as their potential detection in food matrices.

  • Research Article
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  • 10.1007/s12161-008-9023-6
Detection of Cashew Nut in Foods by a Specific Real-time PCR Method
  • Mar 19, 2008
  • Food Analytical Methods
  • Alexandra Ehlert + 4 more

Appropriate detection methods have to be provided to assure the compliance with the recently established regulatory provisions concerning the labeling of allergens in food. Therefore, a novel real-time polymerase chain reaction (PCR) system for the specific and sensitive detection of cashew nut (Anacardium occidentale) was developed. Specificity was checked against DNA from 56 plant and animal species to avoid cross-reactivity to phylogenetically related and other food-relevant organisms. The absolute limit of detection (LOD) was determined to be 0.5 pg genomic cashew DNA and 10 copies, respectively, and the practical LOD examined exemplarily for pesto Genovese was 2 mg/kg. In addition, analysis of different retail samples was performed to demonstrate the suitability of the new assay for manifold applications.

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  • Research Article
  • Cite Count Icon 4
  • 10.5539/jfr.v8n3p71
Evaluation of a Real Time PCR Assay and a ELISA Method for the Detection of Walnuts and Almonds Allergen Traces in Food Products
  • Apr 16, 2019
  • Journal of Food Research
  • Dimitra Houhoula + 9 more

Food allergens are a well acknowledged issue in food industry and are regulated by legislation. The presence of allergens can either origin from the raw material or due to contamination during production. Allergen information on packaging is mandatory although it cannot be accurate in the case of contamination therefore warnings are used. The purpose of the study is the development and validation of a SYBR Green Real Time Polymerase Chain Reaction method using specific primer pairs based on Jug r 1, Jug r 3, and Jug r 4 allergen-coding sequences to improve the sensitivity of Real Time Polymerase Chain Reaction techniques for detection of walnut and almond traces in commercial food products and its comparison with ELISA methodology in terms of detection ability. A total of 100 samples were collected from local markets and were analyzed by Real Time Polymerase Chain Reaction (RT-PCR) and ELISA methods. The results indicated that 16 samples (16%) were found positive in walnut traces and 18 samples (18%) were found positive in almond traces by Real Time Polymerase Chain Reaction of which Elisa identified 14 samples (14%) positive in walnut traces and 15 samples (15%) positive in almond traces. Among them, 4 samples (25%) that contained walnut traces and 6 samples (33.3%) that contained almond traces had no allergen declaration on their label. The improved accuracy of Real Time Polymerase Chain Reaction underlines the importance of this method for allergen detection and quantification in the food industry

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