Abstract
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Highlights
The objective of this study was to evaluate the follicular population on ovaries as related to the phases of oestrous cycle and age in bitches
Medium immunoreaction was seen in secondary follicles and low expression of oestrogen receptor alpha was in tertiary follicles
Diameters of follicles increased synchronously to follicle development (Table 1) and diameters of oocytes in tertiary follicles were higher in comparison with oocytes in secondary follicles (Table 2)
Summary
The objective of this study was to evaluate the follicular population on ovaries as related to the phases of oestrous cycle and age in bitches. The numbers and proportions of primary, secondary, tertiary and atretic follicles, diameters of the follicles and oocytes and distribution of oestrogen receptor alpha in ovaries after ovariectomy were evaluated. Proportions of primary, secondary and tertiary follicles regardless of phase of the oestrous cycle as well as age of animals were 72.0%, 24.5% and 3.5%, respectively. The study did not show an effect of oestrous cycle and age on proportions of primary, secondary, tertiary and atretic follicles as well as oestrogen receptor alpha distribution on ovaries of the bitch. Object of this study was the occurrence of ovarian follicles in various developmental stages in young and old bitches as well as in various phases of oestrous cycle, diameters of the follicles and oocytes and distribution of oestrogen receptor alpha in canine ovaries
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