Abstract
Polycystic ovary syndrome (PCOS) is a common complex and heterogeneous disorder, affecting up to 10% women at reproductive age. It causes three fourth of the ovulatory infertility and PCOS patients often give poor IVF quality. Although some metabolic profiles have been investigated in PCOS patient sera and urine, the follicular fluid, providing fruitful biochemical information about oocyte environment during development has been ignored. In this work, based on NMR metabolomics approach, metabolic profile of follicular fluid of PCOS patients has been explored and compared with healthy controls. Significant increases of glycoprotein, acetate, cholesterol, significant decreases of lactic acid, glutamine, pyruvate, and alanine, have been discovered in PCOS follicular fluids. Furthermore, the Pearson correlations analysis indicated significant relationship existed between ART results and NMR detected follicular metabolites. All these results indicated that PCOS may induce dyslipidemia, low-grade inflammation, and disorder of glycolysis, pyruvate and amino acid metabolism in follicular fluids.
Highlights
Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder affecting 5~10% of women at their reproductive age [1, 2]
All these results indicated that PCOS may induce dyslipidemia, low-grade inflammation, and disorder of glycolysis, pyruvate and amino acid metabolism in follicular fluids
The 1H NMR CPMG spectra averaged over follicular fluid samples from each of the PCOS and control cohorts, together with the average difference spectrum, were shown in Supplementary Figure 1
Summary
Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder affecting 5~10% of women at their reproductive age [1, 2]. The clinical manifestations of this highly heterogeneous disease include amenorrhea, hirsutism, obesity, hyperinsulinemia, hyperandrogenism, polycystic ovaries via ultrasound, and it attributes three fourth of the ovulatory infertility [3]. Oocytes collected from PCOS patients who undergo IVF are often of poor quality, leading to a high cancelation rate and low fertilization rate [5, 6]. The main underlying pathophysiological mechanism was thought to be the abnormally increased androgen and/or insulin level. The obesity, in another way, performs as a synergistic effect in PCOS patients [7]. The complex etiology of PCOS, as well as how PCOS affects oocytes development, relating to both environmental and genetic factors, has not been fully understood yet
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
