Folic acid loaded lipid nanocarriers with promoted skin antiaging and antioxidant efficacy

Abstract

Ageing is the progressive deterioration of physiological functions in organisms. Folic acid (FA) can be used as antiaging. The objective of this research is the feasibility of topical delivery of FA using nanostructured lipid carriers (NLCs) to offer sustained antioxidant and antiaging effects. FA NLCS were prepared by hot high pressure homogenization. Encapsulation efficiency (EE), in vitro release, ex vivo permeation and skin targeting were evaluated. Characterization of selected formulations was assessed, together with stability and water occlusion. Further, histology and antioxidant potential were evaluated. EE exceeded 95%. Formulations with 30% oil showed maximum skin targeting, water occlusion and high efficiency of hydration and antioxidant potential. Incorporation of oil into solid lipids causes more imperfections in the crystal lattice, providing high drug encapsulation. The small particle size leads to forming a depot in the skin providing efficient skin targeting, water occlusion, antiaging and antioxidant effects especially for the 30% oil containing formula; F11. The results emphasize the success of topical application of FA NLCs. F11 represents a promising, stable preparation, achieving remarkable long acting skin hydration, in vitro and ex vivo antioxidant potential lasting for >4 days.