Folic Acid-Adorned Curcumin-Loaded Iron Oxide Nanoparticles for Cervical Cancer.

Marzieh Ramezani Farani,Mohammad Reza Saeb,Hamid Heydari Sheikh Hossein,Seyyedeh Maedeh Mousavi,Maryam Azarian,Pooyan Makvandi,Arash Moradi,Milad Ashrafizadeh,Zahra Mohammadi Abgarmi,Navid Rabiee,Zohreh Abdolvahabi

doi:10.1021/acsabm.1c01311

Abstract

Cancer is a deadly disease that has long plagued humans and has become more prevalent in recent years. The common treatment modalities for this disease have always faced many problems and complications, and this has led to the discovery of strategies for cancer diagnosis and treatment. The use of magnetic nanoparticles in the past two decades has had a significant impact on this. One of the objectives of the present study is to introduce the special properties of these nanoparticles and how they are structured to load and transport drugs to tumors. In this study, iron oxide (Fe3O4) nanoparticles with 6 nm sizes were coated with hyperbranched polyglycerol (HPG) and folic acid (FA). The functionalized nanoparticles (10–20 nm) were less likely to aggregate compared to non-functionalized nanoparticles. HPG@Fe3O4 and FA@HPG@Fe3O4 nanoparticles were compared in drug loading procedures with curcumin. HPG@Fe3O4 and FA@HPG@Fe3O4 nanoparticles’ maximal drug-loading capacities were determined to be 82 and 88%, respectively. HeLa cells and mouse L929 fibroblasts treated with nanoparticles took up more FA@HPG@Fe3O4 nanoparticles than HPG@Fe3O4 nanoparticles. The FA@HPG@Fe3O4 nanoparticles produced in the current investigation have potential as anticancer drug delivery systems. For the purpose of diagnosis, incubation of HeLa cells with nanoparticles decreased MRI signal enhancement’s percentage and the largest alteration was observed after incubation with FA@HPG@Fe3O4 nanoparticles.

Highlights

The fourth most common malignant gynecological tumor in women is cervical cancer
An increase in the peak rate of O−H demonstrates the increased hydrophilicity of hyperbranched polyglycerol (HPG)-coated nanoparticles treated with folic acid (FA).[67]
The current study describes the synthesis and characterization of small and stable magnetic Iron oxide nanoparticles (IONPs), with narrow size distribution, and complexed with HPG hydroxyl groups using the polyol method

Summary

Introduction

The fourth most common malignant gynecological tumor in women is cervical cancer. In 2018, cervical cancer caused 311,000 deaths from 570,000 diagnosed cases.[1]. The incidence rate of cervical cancer has demonstrated a decrease in recent years, but distant-stage disease and cervical adenocarcinoma are a threat to the life of many young women around the world, and these cannot often be diagnosed by cytology.[2] Cervical cancer is more common in developing countries compared to developed countries.[3] In addition to cytology for detection of cervical cancer, there have been preventative measures in which vaccination against human papillomavirus is the most important.[4] cervical cancer is still a leading cause of death among women and new therapeutic strategies should be designed for its treatment.[5] Nanotechnological approaches including the use of microfluidic devices, high-gravity techniques, microporous smart nanostructures, green methodologies, and Received: December 27, 2021 Accepted: February 7, 2022

Objectives

Methods

Results

Conclusion