Foliar Soil and Agricultural Application of Gibberellic Acid to Alleviate the Effect of Nickel Sulphate Stress Brassica napus L

Abstract

There are 150 species of the genus brassica that are grown globally as oilseed crops or vegetables. Mustard plants belong to the family brassicaceae. Oil is obtained from mustard seeds, their leaves are edible and can be consumed as mustard greens. Gibberellic acid (GA) is a hormone or growth regulator present in variable amounts in all sections of plants. The presence of heavy metals has turned into a problem because of their harmful results on plants even at low concentration. The present study comprises of a pot experiment that was perform at the Old Botanical Garden, University of Agriculture Faisalabad to examine the effect of exogenous supplication of gibberellic acid on development and various morphological parameters of mustard (Brassica napus L.) within NiSo4 tensity on two varieties (super canola and sherilla). The experiment was designed CRD (completely randomized design) by factorial arrangement and 3 replications. A 300 µM nickel sulphate stress was applied and to overcome the effect of metal stress a foliar application of (0.25 mM) gibberellic acid was applied. The samples were dividing into two different sampling bags with three replicates. Plastic zipper bags containing fresh samples were kept in the freezer for different biochemical analysis like chlorophyll and carotenoids and the data were collected for the determination of various morpho-physiological parameters. While the remaining samples were placed in the oven for dry analysis for samples Na+, K+ and Ca+. These parameters were analyzed by using CO-STAT software program.