Foliar-selenium enhances plant growth and arsenic accumulation in As-hyperaccumulator Pteris vittata: Critical roles of GSH-GSSG cycle and arsenite antiporters PvACR3

Abstract

It is known that selenium (Se) enhances plant growth and arsenic (As) accumulation in As-hyperaccumulator Pteris vittata, but the associated mechanisms are unclear. In this study, P. vittata was exposed to 50 μM arsenate (AsV) under hydroponics plus 25 or 50 μM foliar selenate. After 3-weeks of growth, the plant biomass, As and Se contents, As speciation, malondialdehyde (MDA) and glutathione (GSH and GSSG) levels, and important genes related to As-metabolism in P. vittata were determined. Foliar-Se increased plant biomass by 17 − 30 %, possibly due to 9.1 − 19 % reduction in MDA content compared to the As control. Further, foliar-Se enhanced the As contents by 1.9–3.5 folds and increased arsenite (AsIII) contents by 64 − 136 % in the fronds. The increased AsV reduction to AsIII was attributed to 60 − 131 % increase in glutathione peroxidase activity, which mediates GSH oxidation to GSSG (8.8 −29 % increase) in the fronds. Further, foliar-Se increased the expression of AsIII antiporters PvACR3;1–3;3 by 1.6 − 2.1 folds but had no impact on phosphate transporters PvPht1 or arsenate reductases PvHAC1/2. Our results indicate that foliar-Se effectively enhances plant growth and arsenic accumulation by promoting the GSH-GSSG cycle and upregulating gene expression of AsIII antiporters, which are responsible for AsIII translocation from the roots to fronds and AsIII sequestration into the fronds. The data indicate that foliar-Se can effectively improve phytoremediation efficiency of P. vittata in As-contaminated soils.