Foliar nitrogen metabolism of adult Douglas-fir trees is affected by soil water availability and varies little among provenances.

Baoguo Du,Kirstin Jansen,Ulrich Kohnle,Laura Verena Junker,Jürgen Kreuzwieser,Arthur Gessler,Henning Wildhagen,Monika Eiblmeier,Heinz Rennenberg,Ingo Ensminger,Anita Kleiber,Michael Dannenmann,Moritz Hess,Berthold Heinze

doi:10.1371/journal.pone.0194684

Abstract

The coniferous forest tree Douglas-fir (Pseudotsuga menziesii) is native to the pacific North America, and is increasingly planted in temperate regions worldwide. Nitrogen (N) metabolism is of great importance for growth, resistance and resilience of trees. In the present study, foliar N metabolism of adult trees of three coastal and one interior provenance of Douglas-fir grown at two common gardens in southwestern Germany (Wiesloch, W; Schluchsee, S) were characterized in two subsequent years. Both the native North American habitats of the seed sources and the common garden sites in Germany differ in climate conditions. Total and mineral soil N as well as soil water content were higher in S compared to W. We hypothesized that i) provenances differ constitutively in N pool sizes and composition, ii) N pools are affected by environmental conditions, and iii) that effects of environmental factors on N pools differ among interior and coastal provenances. Soil water content strongly affected the concentrations of total N, soluble protein, total amino acids (TAA), arginine and glutamate. Foliar concentrations of total N, soluble protein, structural N and TAA of trees grown at W were much higher than in trees at S. Provenance effects were small but significant for total N and soluble protein content (interior provenance showed lowest concentrations), as well as arginine, asparagine and glutamate. Our data suggest that needle N status of adult Douglas-fir is independent from soil N availability and that low soil water availability induces a re-allocation of N from structural N to metabolic N pools. Small provenance effects on N pools suggest that local adaptation of Douglas-fir is not dominated by N conditions at the native habitats.

Highlights

Douglas-fir (Pseudotsuga menziesii) is native to America’s Pacific Northwest (USA, Canada), where it grows in more than 20 million ha of natural forests [1]
N metabolism of field grown adult Douglas-fir modelling revealed that total available soil water (TAW) was an important factor (Table 5) underlying variation in N pools between sites indicated by the exploratory analysis (Fig 1 and S1 Fig)
These results show that responses to low TAW were not limited to N metabolism

Summary

Introduction

Douglas-fir (Pseudotsuga menziesii) is native to America’s Pacific Northwest (USA, Canada), where it grows in more than 20 million ha of natural forests [1]. Reflecting the great natural range of distribution in longitude, latitude and altitude (from California to British Columbia, and from sea level up to an altitude of >2,000 m), a variety of different ecotypes evolved. These ecotypes are locally adapted, i.e. phenotypes of adaptive traits vary in a way providing highest fitness in the native environments [2,3,4]. Variation in N availability may cause local adaptation at the level of N uptake [22,23] or metabolism, which is reflected in constitutive variation in N pools among provenances as reported for Norway spruce [20] and Scots pine [24,25]. This, in turn, might enable higher net C assimilation rates, which might compensate for environmental limitations for carbon gain

Methods

Results

Discussion

Conclusion