Foliar application of molybdenum in common bean. II. Nitrogenase and nitrate reductase activities in a soil of low fertility

Abstract

Foliar application of molybdenum (Mo) at 40 g ha‐1 25 days after plant emergence greatly enhanced nitrogenase and nitrate reductase activities of common bean (Phaseolus vulgaris L.), resulting in an increase in total nitrogen (N) accumulation in shoots. Application of 20 kg N ha‐1 as ammonium sulfate [(NH4)2SO4] at sowing decreased nodulation and nitrogenase activity. Rhizobium inoculation did not affect nitrogenase activity which demonstrated that Mo application increased the efficiency of native Rhizobia strains. Nitrogen amendment, either at planting (20 kg N ha‐1) or as a side dressing (30 kg N ha‐1) 25 days after plant emergence, did not affect the foliar nitrate reductase activity. Molybdenum foliar spray as ammonium molybdate [(NH4)6Mo7O242H2O] and N applied as a side dressing increased equally the total amount of N in the pods. A 10% increase in the seed N concentration was obtained with foliar application of Mo, while N applied as a side dressing had no effect on seed N concentration. An average increase of 41% in N export to the seeds was obtained by either Mo or N as side dressing. Nitrogen applied at sowing or Rhizobia inoculation had no effect on the characteristics evaluated 74 days after plant emergence. Plants that received either Mo as foliar spray or as side dressed N had similar yields. This demonstrated that, in certain soils, N fertilization may be replaced by a small amount of Mo as a foliar application.