Abstract

Folded chromosomes of haploid a or diploid aa cells arrested by α-factor are characterized by a heterogeneous sedimentation distribution, distinguishable from the corresponding g 0and g 1 structures. The extent of heterogeneity appears to depend in part on the extent of the characteristic schmoe morphology. Under conditions where a and α mating-type cells are mixed together without conjugation, folded chromosomes characteristic of α-factor arrested a and aa cells appear in both cell types in a synchronous manner. Attempts to order G0 and α-factor arrest suggest that these two arrest stages represent two different branch pathways out of the cell cycle. These two branch pathways may have a common branch point, or they may be characterized by two different branch points.

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