Abstract
Analysis of folded chromosomes in cells under standard sporulation conditions shows that the g 0 form of the folded genome is used as the entry into meiosis. Premeiotic DNA replication is initiated from the g 0 structure. In contrast, mitotic DNA replication is preceded by a characteristic pre-replicative form, g 1. Nonetheless, the mitotic and meiotic replication structures are indistinguishable by sedimentation. Preliminary evidence also suggests that the meiotic equivalent of the mitotic post-replicative structure, g 2, is absent. In strains homozygous for the mating type locus, aa and αα, meiotic replicating structures are not detected, and the folded chromosomes remain in a non-cycling form. However, this non-cycling form is distinguishable from the g 0 form of aα. cells.
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