Abstract
We propose a method for microscope point spread function computation in which both design and actual acquisition parameters are explicitly introduced in the integrals describing the electromagnetic field in the focal region. This model therefore combines the ease of use of the Gibson and Lanni scalar approach with the accuracy of the Torok and Varga method. We also compare some theoretical predictions of this model with those of a scalar model. In particular, the scalar model underestimates the point spread function size. This has practical application, for example when deconvolving microscope images or analyzing point spread functions. The method may also be used for confocal microscopy.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
