Foams Made of Engineered Recombinant Spider Silk Proteins as 3D Scaffolds for Cell Growth

Abstract

Materials for tissue engineering have to be biocompatible and have to support cell adhesion, proliferation and differentiation. Additionally, in case of soft tissue engineering the mechanical properties have to accommodate that of the tissue with mechanical integrity until the artificial scaffold is replaced by natural extracellular matrix. In case of artificial 3D scaffolds, it is of critical importance to be able to tune the mechanical properties, the inner free volume (i.e., pore size) and degradation behavior of the employed biomaterial. Here, the potential of recombinant spider silk proteins was evaluated concerning their processing into and application as 3D scaffolds for soft tissue engineering. Highly porous foams made of the recombinant spider silk protein eADF4(C16) and a variant containing an RGD motif were fabricated by salt leaching yielding mechanically robust scaffolds. In contrast to other salt-leached silk scaffolds, the swelling behavior of these scaffolds was low, and the mechanical properties in the range of soft tissues. The pore size and porosity of the foams could be adjusted by the salt crystal size. Fibroblasts adhered and proliferated well in foams made of the spider silk RGD variant but not in the foams of the nonmodified one.