Abstract
The fibronectin type III domain-containing protein 5 (FNDC5) discovered in 2002 has recently gained attention due to its potential role in protecting against obesity. In rat, no data exist regarding FNDC5 production and regulation in the stomach. The aim of the present work was to determine the expression of FNDC5 in the rat stomach and its potential regulation by body composition. The present data shows FNDC5 gene expression in the gastric mucosa. Immunohistochemical studies found FNDC5 immunopositivity in chief cells of gastric tissue. By the use of three different antibodies FNDC5 was found expressed in gastric mucosa and secreted by the stomach. The rate of gastric FNDC5 secretion parallels the circulating levels of FNDC5. The body fat mass increase after intervention with high fat diet coincided with a decrease in the secretion of FNDC5 from the stomach and a diminution in the FNDC5 circulating levels. In summary, the present data shows, for the first time, the expression of FNDC5 in the stomach of rats and its regulation by body composition, suggesting a potential role of gastric FNDC5 in energy homeostasis.
Highlights
Obesity represents a major public health problem in developed countries
The rate of fibronectin type III domain-containing protein 5 (FNDC5) gastric secretion was related with its circulating levels and with PGC1 expression in brown adipose tissue (BAT)
As previously reported[1,3,24,25,26], the gastrointestinal tract is crucial for energy homeostasis regulation
Summary
The most effective treatment for this pathology is gastric surgery[1,2] This finding suggests that signals from the gastrointestinal tract are crucial for the regulation of energy balance[3,4,5]. Others reported an inverse relationship between circulating irisin levels and obesity[18,19] or no correlation with BMI20. It was shown that irisin levels dropped after six months post-surgery in obese patients who had undergone bariatric surgery[9]. This finding might suggest that in humans the FNDC5/irisin produced by the stomach would contribute to the circulating irisin levels. Gastric FNDC5/irisin production in rat stomach has not yet been assayed
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