FlyDriver: A Connectomic Approach to Find Specific Drivers of Target Neuron in the FlyCircuit

Abstract

Event Abstract Back to Event FlyDriver: A Connectomic Approach to Find Specific Drivers of Target Neuron in the FlyCircuit Ting-Yuan Wang1, Yen-Jen Lin2, Hsuan-Wen Lin1, Chao-Chun Chuang3, Meng-Hsuan Chiang1 and Ann-Shyn Chiang1, 2, 4, 5* 1 National Tsing Hua University, Institute of Biotechnology, Taiwan 2 National Tsing Hua University, Brain Research Center, Taiwan 3 National Center for High-performance Computing, Taiwan 4 Academia Sinica, Genomics Research Center, Taiwan 5 University of California at San Diego, Kavli Institute for Brain and Mind, United States We collected about 4000 driver expression images of Drosophila melanogaster, which included Gal4, LexA, flipase and spilt-Gal4 system for manipulating neuronal circuit function. All confocal microscopy images was archived into a database, named FlyDriver. FlyDriver was followed the same platform of FlyCircuit. Therefore, we can compare single neuron image with driver image. Here, we used image-matching algorithm on both neuron-driver image pairs and driver-driver image pairs between FlyCircuit and FlyDriver, which amount over the 100 million image pairs was been calculated. The overlap information of neuron-driver image pairs could help user to find a specific driver with a target neuron. In addition, user could also find similar driver with overlap information of driver-driver image pairs. We have illustrated the utility of these image-matching data for identifying the putative driver expressed target neuron and finding the specific driver from a set of neuron images. For some expression pattern that did not exist in one single driver, we could use the overlap information to predict expression patterns that comes from the intersection region from two driver images. As a result, FlyDriver provided a web interface that allows users to find the driver with different criterion. This will facilitate finding a specific driver to understanding the function of neuronal circuit. Keywords: neuron image, driver image, image databse, driver database, Image matching algorithm Conference: Neuroinformatics 2015, Cairns, Australia, 20 Aug - 22 Aug, 2015. Presentation Type: Poster, to be considered for oral presentation Topic: Computational neuroscience Citation: Wang T, Lin Y, Lin H, Chuang C, Chiang M and Chiang A (2015). FlyDriver: A Connectomic Approach to Find Specific Drivers of Target Neuron in the FlyCircuit. Front. Neurosci. Conference Abstract: Neuroinformatics 2015. doi: 10.3389/conf.fnins.2015.91.00011 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 May 2015; Published Online: 05 Aug 2015. * Correspondence: Prof. Ann-Shyn Chiang, National Tsing Hua University, Institute of Biotechnology, Hsinchu city, 300, Taiwan, aschiang@life.nthu.edu.tw Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ting-Yuan Wang Yen-Jen Lin Hsuan-Wen Lin Chao-Chun Chuang Meng-Hsuan Chiang Ann-Shyn Chiang Google Ting-Yuan Wang Yen-Jen Lin Hsuan-Wen Lin Chao-Chun Chuang Meng-Hsuan Chiang Ann-Shyn Chiang Google Scholar Ting-Yuan Wang Yen-Jen Lin Hsuan-Wen Lin Chao-Chun Chuang Meng-Hsuan Chiang Ann-Shyn Chiang PubMed Ting-Yuan Wang Yen-Jen Lin Hsuan-Wen Lin Chao-Chun Chuang Meng-Hsuan Chiang Ann-Shyn Chiang Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.