Abstract
A very rapid and sensitive fluorescence technique has been described for detecting and quantitatively assaying proteins in polyacrylamide gels. Proteins were labeled with fluorescamine, subjected to SDS polyacrylamide gel electrophoresis and scanned for fluorescence with a modified Gilford 2410-S linear transport. The fluorescence signal was linear with the concentration of protein over a range of 0.5–7 μg (myoglobin), 9 μg (chymotrypsinogen A), and at least 12.5 μg (ovalbumin). The limits of sensitivity were not determined but as little as 6 ng myoglobin could be measured accurately. Several applications for fluorescence gel scanning were discussed.
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