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Abstract
A flow injection system based on the p-hydroxyphenylacetate-peroxide-peroxidase reaction allows the simultaneous determination of H/sub 2/O/sub 2/ and CH/sub 3/HO/sub 2/ at 50 samples/h with an LOD of 0.1 /sup +/g/L (3 nM) H/sub 2/O/sub 2/. A pressurized porous PTFE membrane reactor introduces the enzyme, and the pH of the flow stream is altered by introducing NH/sub 3/ through a nonporous cation exchange membrane reactor. Excellent reproducibility, precision, and sensitivity are achieved with the membrane reactors and an inexpensive filter fluorometer with a monochromatic excitation source. Low levels of residual peroxide in water are ubiquitous; such levels return soon after the H/sub 2/O/sub 2/ is catalytically removed.
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