Abstract
This report describes a microplate-based enzyme immunoassay with fluorometric detection (FEIA) for the determination of thyroxine (T4) from dried blood spots. The T4 FEIA was developed for newborn screening of congenital hypothyroidism (CH) and for the follow-up of l-thyroxine substitution therapy in CH patients. The detection limit of the proposed method was 8.0 nmol/l blood, with imprecision being from 5.1 to 11.9% (within-run) and from 4,6% to 9.8% (between-run). Highly significant but moderate correlation was observed with the Neonatal T4 RIA of Diagnostic Products ( r=0.72, P<0.0005; n=47) and the Spectria R serum T4 RIA of Orion ( r=0.79, P<0.0005; n=89). The overall incubation time of the assay is 3 h. The assay uses the same fluorogenic substrate as the neonatal thyrotropin (TSH) test described earlier [14], thus allowing simultaneous determination of both T4 and TSH.
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