Abstract
A highly sensitive method has been developed to measure quantitatively the DNA content of yeast cells. The reaction between the fluorescent dye 4′, 6-diamidino-2-phenyl-indol-dihydrochloride and DNA in a crude lysate from protoplasts can be measured without prior extraction of the DNA. Interferences by the high protein and RNA content of yeast cells, which often limit the accuracy of common methods employed in the determination of DNA content, were eliminated by suitable enzymatic pretreatment of the cell lysate.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
