Abstract
A sensitive fluorometric method for the determination of primary aromatic amines is described. The procedure consists of diazotization of the amino group followed by coupling with 4-methoxy-m-phenylenediamine (MPD), and reaction of the resulting azo compound with ammoniacal cupric sulfate. By this reaction, primary aromatic amines are derivatized to intensely fluorescent compounds, and a method for the determination of p-aminobenzoic acid (PABA) was established. A linear relationship was observed between the PABA concentration and the fluorescence intensity in the range of 0.001-1.0 μg/ml. The proposed method is applicable to the determination of other primary aromatic amines.
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