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Abstract
To obtain suitable conditions for the simultaneous determination of primary and secondary amines by high-performance liquid chromatography with post-column derivatization, the conversion of secondary amines into primary amines with sodium hypochlorite was reinvestigated by flow injection analysis. While NaOCl was required primarily for the conversion of secondary amines into primary amines, it also decreased the detectability of the latter with o-phthalaldehyde-2-mercaptoethanol reagent, depending on the reaction on the reaction temperature and time. The conditions established for the post-column derivatization were applied to the determination of amino acids including l-proline, l-4-hydroxyproline and N-methyl amino acids, catecholamines and their 3-O-methyl derivatives.
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