Abstract
The high-performance liquid chromatography (HPLC) method for detecting prostaglandins (PGs) and thromboxane (TX) which was reported previously was found to have a serious problem in the detection of PGs and TX. In the present study, we tried to solve this problem by developing a sensitive HPLC method for determining PGs and TX in human plasma. An analytical wavelength (412nm when excited at 350nm) which was not interfered with by the Raman spectrum was found to be suitable for the detection of PGs- and TX-ADAM (9-anthryldiazomethane) derivatives. Moreover, partial purification of 9-anthyryldiazomethane (ADAM) derivatives by using a GEL PACK-A110 column enabled us to remove interfering substances from the chromatogram. The levels of 6-Keto-PGF1, PGE2 & D2, PGF2 and TXB2 in plasma were detected simultaneously using our method. The present HPLC method is useful for determining the PGs and TX levels in biological samples.
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Schedule a callMore From: Nihon eiseigaku zasshi. Japanese journal of hygiene
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