Abstract
Ampicillin forms a strongly fluorescent yellow product in acid solution during hydrolysis at elevated temperature. The quantity and rate of formation of the fluorescent material are enhanced appreciably by formaldehyde, and the fluorescent product exhibits extraction properties of an organic acid. In alkaline solution, the fluorescent compound has uncorrected excitation and emission maxima at 346 and 422 nm., respectively. An assay procedure based on these observations permits detection of less than 0.05 mcg./ml. of ampicillin and/or α-aminobenzylpenicilloic acid. By combining fluorometric and microbiological methods, measurement can be made of ampicillin degradation products as well as the unchanged antibiotic in serum and urine.
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