Abstract
A sensitive and selective method for the determination of diethylstilbestrol (DES) by fluorescence immunoassay (FIA) was established on the basis of competitive immunoreaction. The complex of avidin conjugated by quantum dots (QDs-avidin) was regarded as a probe in this system and the avidin-biotin system as the signal amplification system. After optimizing the conditions of immunoreaction such as the time and temperature of incubating, the pH of the buffer solution, the concentration of the reagent and so on, the linear range and the limit of detection of DES were 0.01-2000 ng·mL-1 and 4.5 pg·mL-1, respectively. The method was applied to determine DES in flesh of chicken, with the percent recoveries in the range of 81.0%-109.8%.
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