Abstract
Rapid analyte measurement platform (RAMP™) fluorogenic hand‐held immunoassays (HHAs) were evaluated for inclusivity/sensitivity, exclusivity/specificity, sample matrix effects, ruggedness/stability, and reproducibility in detection of ricin (RCA60), a potential biological threat agent. The limit of detection of HHAs for RCA60 was 14 ng/mL or approximately 140 pg/test. HHAs were inclusive in detection of ricin RCA60, RCA120, ricin A chain, and ricin B chain and exclusive in discrimination of RCA60 from other toxins. None of the sample matrices tested affected assay performance. RAMP™ ratios were tolerant of increases in sample volume, however, a decrease in sample volume of 25% resulted in significantly increased readings. RAMP™ readings were highly reproducible lot‐to‐lot, cartridge‐to‐cartridge, day‐to‐day, and reader‐to‐reader.
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