Abstract
Mesoporous silica nanoparticles loaded with rhodamine B and capped with curcumin are used for the selective and sensitive fluorogenic detection of human serum albumin (HSA). The sensing mesoporous silica nanoparticles are loaded with rhodamine B, decorated with aminopropyl moieties and capped with curcumin. The nanoparticles selectively release the rhodamine B cargo in the presence of HSA. A limit of detection for HSA of 0.1 mg/mL in PBS (pH 7.4)-acetonitrile 95:5 v/v was found, and the sensing nanoparticles were used to detect HSA in spiked synthetic urine samples.
Highlights
Human serum albumin (HSA) is a vital protein that constitutes around 50% of the total proteins in human plasma [1]
HSA is synthesized in the liver, and it is related with the transport of several endogenous and exogenous biomolecules such as fatty acids, thyroxine, hormones and drugs [2]
Data analysis was based on the Mie theory using refractive indices of 1.33 and 1.45 for the dispersant and mesoporous silica nanoparticles, respectively
Summary
Human serum albumin (HSA) is a vital protein that constitutes around 50% of the total proteins in human plasma [1]. HSA is synthesized in the liver, and it is related with the transport of several endogenous and exogenous biomolecules such as fatty acids, thyroxine, hormones and drugs [2]. HSA plays a vital role in the regulation of plasma osmotic pressure, maintain water equilibrium between tissues and preserve blood pH [3]. Normal levels of HSA in serum are in the 35–55 g/L range, whereas in urine its concentration is ca. High levels of HSA in body fluids are related with several human diseases such as kidney failure, diabetes mellitus, cardiovascular disorders, obesity and liver injury [4]. Low HSA levels are found to cause chronic hepatitis, liver failure and cirrhosis [5]
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