Abstract
Abstract The precision limit of visual comparison procedures used in aflatoxin assay methods can be no better than ± 2 0 % for a single observation and, under operating conditions, it is probably close to ± 2 8 % . Fluorodensitometric procedures described in the literature can improve this precision to ± 9% for the average of multiple observations. A possible source of inaccuracy in the published densitometric procedures is pointed out and a method using internal standards to avoid the inaccuracy is presented. Tested with spiked extracts of "clean" peanut butter, the procedure gave an average 101 ± 3% recovery of added aflatoxin B1 and 89 ± 6% recovery of aflatoxin B2 with a precision for individual assays equal to the precisions reported for the other procedures. The major source of error was shown by elimination to reside in the thin layer chromatography and aflatoxin instability.
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