Abstract
Specific labeling of cellular proteins with fluorinated nanobodies enables their visualization in nanoSIMS.
Highlights
Selda Kabatas, *ab Paola Agui-Gonzalez, ab Rena Hinrichs,ab Sebastian Jahne, ab Felipe Opazo, ab Ulf Diederichsen, c Silvio O
We introduce here new 19F-containing molecules that can be conjugated in a site-specific manner to nanobodies able to recognize fluorescent proteins (FPs) or mouse immunoglobulins (Igs)
We demonstrate that it is possible to use the 19F-nanobodies to reveal the location of several cellular proteins previously tagged with FPs or Igs
Summary
Selda Kabatas, *ab Paola Agui-Gonzalez, ab Rena Hinrichs,ab Sebastian Jahne, ab Felipe Opazo, ab Ulf Diederichsen, c Silvio O. We demonstrate that it is possible to use the 19F-nanobodies to reveal the location of several cellular proteins previously tagged with FPs or Igs. This enables specific bio-imaging in SIMS for a vast repertoire of biomolecules, offering new opportunities to study specific structural and functional molecular interactions in biological specimens.
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