Abstract
Two methods are described for the separation of acridine from organic airborne particulates or coal-tar-pitch. A basic fraction was not necessary for these analyses. With the help of instant thin-layer chromatography (ITLC) with glass-fiber paper impregnated with silica gel, acridine was separated in 18 minutes from the tens of thousands of other compounds in the sample. Separation by paper electrophoresis required about 100 minutes, but the separation was sharper in that acridine and an alkylacridine were separated from each other and also from the quinolinic compounds above them, the larger aza arenes below them, and the innumerable non-basic compounds at the origin. After separation, the samples were assayed by scanning the standard and “unknown” acridine spots at F 345/475. Although the electrophoretic method requires more time, it is superior to the ITLC method for estimation of acridine in samples from urban air and from source effluents.
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