Fluorimetric determination of tetracyclines with the europium chelate of 1,10-phenanthroline in micellar solutions of anionic surfactants

Abstract

The fluorescence of europium(III) in micellar solutions of surfactants sensitized by tetracycline derivatives was studied. It was found that the fluorescence intensity in the system Eu3+-tetracycline derivatives increases in the presence of 1,10-phenanthroline by a factor of 3–4. In micellar solutions of sodium dodecylbenzene sulfonate, the signal additionally enhances by a factor of 2.5. A procedure was developed for determining tetracycline and three its derivatives in blood plasma.