Abstract
A modified spectrofluorimetric method for the determination of selenium in some marine tissues is described. Selenium is isolated from potentially interfering elements by co-precipitation, then a piazselenol is formed between selenium and 2.3-diaminonaphthalene at pH 1.0 and extracted into cyclohexane. The selenium is determined fluorometrically (λ ex, 377 nm; λ em, 519 nm) in the extract. The detection limit is 3.6 rig (6 times the standard deviation of the blank) and the coefficient of variation is 4% at the 10-ng level.
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