Fluorimetric determination of isoxsuprine hydrochloride in pharmaceuticals and biological fluids

Abstract

Two simple and highly sensitive fluorimetric methods have been developed for the determination of isoxsuprine hydrochloride in bulk, in dosage forms and in biological fluids. The first method involves the direct measurement of the native fluorescence of the drug in the concentration range 0.4–4.0 μg ml −1, the second method is based on the oxidation of isoxsuprine HCl with cerium(IV) followed by fluorimetric measurement in the concentration range 0.02–0.2 μg ml −1. The average % found were 99.9±0.78 and 100.0±0.62 for the two methods, respectively. The minimum detectability (3 S B) were 0.11 and 0.007 μg ml −1 for the two methods, respectively. The methods results showed insignificant difference with those of the official method.