Abstract
A selective and sensitive assay of hydroxyproline in blood plasma is presented. The technique uses ion exchange chromatography followed by reaction with 7-chloro-4-nitrobenzofurazan (NBD chloride) and continuous recording of the resulting fluorescence ( λ ex = 465 nm, λ f1 = 535 nm). Proline and prolyland hydroxyprolyl-peptides can also be measured by this system. For hydroxy-proline, the method is more sensitive than procedures using ninhydrin or p-dimethylaminobenzaldehyde.
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