Abstract
A simple, sensitive, and selective fluorimetric method is reported for the determination of β-carotene in various food samples. Three fluorescent dyes, including fluorescein (F), eosin B (EB), and Congo red (CR), were characterized in a series of measurements to optimize the analytical response for the reliable, suitable, and sustainable determination of β-carotene. 3′,6′-Dihydroxyspiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, commonly known as fluorescein, was selected for the fluorimetric determination of β-carotene using a phosphate buffer. Under the optimum experimental conditions, β-carotene was determined across a range from 0.54 to 536.87 mg L−1 with a detection limit equal to 0.47 mg L−1. The novel method was demonstrated to be easy to use, cost effective, rapid, and less complex compared to a standard protocol that involves a time-consuming extraction followed by an expensive HPLC determination.
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