Abstract
Abstract A novel and simple method for determining microsomal lauric acid hydroxylase activity is presented. Lauric acid and hydroxy-metabolites are separated using differential acid/base solubilities coupled to solvent partitioning. After esterification with 1-pyrenyldiazomethane, metabolites are quantitated using isocratic high-performance iiquid chromatography with fluorimetric detection. Column washing and equilibration between samples is not required. The method was verified by measuring the induction, in rats, of microsomal lauric acid hydroxylase activity by clofibrate. The method has clear advantages over published radiochemical procedures for measuring the formation of hydroxylated metabolites of lauric acid.
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