Abstract
Abstract A modification of the spectrofluorimetric method of Lowe et al 1 for the measurement of glutamic acid decarboxylase (GAD) activity in rat brain is described. This method involves the indirect determination of GAD activity by measurement of the formation of gamma-amino-butyric acid (GABA). The minimum activity of GAD observable was 0.45 μg GABA/hour/mg protein and the limit of detection was 0.20 × 10−6 M GABA. This method was developed for measurement of GAD activity of gabaergic terminals in globus pallidus, endopeduncular nucleus, substantia nigra, and ventromedial thalamus in rat brain.
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